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rabbit polyclonal anti psd 95 antibody  (Proteintech)


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    Structured Review

    Proteintech rabbit polyclonal anti psd 95 antibody
    Rabbit Polyclonal Anti Psd 95 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+psd+95/PSD95-Specific%2CDLG4+Antibody/pm41748571-241-54-60
    Average 96 stars, based on 450 article reviews
    rabbit polyclonal anti psd 95 antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Pearl Powder Exerts Antidepressant Effects by Modulating the GluN2A/BDNF/PSD-95 Signaling Axis
    Article Snippet: .. After blocking the PVDF membrane with blocking buffer (NCM, P30500) for 10 minutes, the membrane was incubated with various primary antibodies respectively (including rabbit anti-PSD-95 (1:1000, Beyotime, AF1096), rabbit anti-GluN2A (1:1000, ABclonal, A0924), rabbit anti-GluN2B (1:1000, Proteintech, AF7029), rabbit anti-BDNF (1:1000, ABclonal, A16299), rabbit anti-Beta Actin (1:5000, Proteintech, 81115-1-RR)) overnight at 4°C (approximately 18 hours). .. The membrane was washed three times with 1×TBST (Solarbio, T1085), 10 minutes for each time.

    Membrane:

    Article Title: Pearl Powder Exerts Antidepressant Effects by Modulating the GluN2A/BDNF/PSD-95 Signaling Axis
    Article Snippet: .. After blocking the PVDF membrane with blocking buffer (NCM, P30500) for 10 minutes, the membrane was incubated with various primary antibodies respectively (including rabbit anti-PSD-95 (1:1000, Beyotime, AF1096), rabbit anti-GluN2A (1:1000, ABclonal, A0924), rabbit anti-GluN2B (1:1000, Proteintech, AF7029), rabbit anti-BDNF (1:1000, ABclonal, A16299), rabbit anti-Beta Actin (1:5000, Proteintech, 81115-1-RR)) overnight at 4°C (approximately 18 hours). .. The membrane was washed three times with 1×TBST (Solarbio, T1085), 10 minutes for each time.

    Incubation:

    Article Title: Pearl Powder Exerts Antidepressant Effects by Modulating the GluN2A/BDNF/PSD-95 Signaling Axis
    Article Snippet: .. After blocking the PVDF membrane with blocking buffer (NCM, P30500) for 10 minutes, the membrane was incubated with various primary antibodies respectively (including rabbit anti-PSD-95 (1:1000, Beyotime, AF1096), rabbit anti-GluN2A (1:1000, ABclonal, A0924), rabbit anti-GluN2B (1:1000, Proteintech, AF7029), rabbit anti-BDNF (1:1000, ABclonal, A16299), rabbit anti-Beta Actin (1:5000, Proteintech, 81115-1-RR)) overnight at 4°C (approximately 18 hours). .. The membrane was washed three times with 1×TBST (Solarbio, T1085), 10 minutes for each time.

    Article Title: FTO (fat-mass and obesity-associated protein) deficiency aggravates age-dependent depression-like behaviors and cognitive impairment
    Article Snippet: .. Rabbit anti-FTO (1:5000, Thermo, USA), rabbit anti-BDNF (1:1000, Cell Signaling Technology, USA), rabbit anti-TrkB (1:1000, Cell Signaling Technology, USA), rabbit anti-PSD-95 (1:5000, Proteintech, USA), rabbit anti-SYN (1:1000, Cell Signaling Technology, USA), rabbit anti-SAP97 (1:1000, Cell Signaling Technology, USA), and mouse anti-tubulin (1:1000, Cell Signaling Technology, USA) were incubated overnight at 4 LJ. .. Subsequently, membranes were incubated with the respective secondary antibodies for 1 hour at room temperature and analyzed using a Bio-Rad ChemiDoc MP Chemiluminescence Gel Imaging System.

    Article Title: Acute elevated dietary fat alone is not sufficient to decrease AgRP projections in the paraventricular nucleus of the hypothalamus in mice
    Article Snippet: .. Slices were then incubated with primary antibodies Rabbit anti-AgRP (Phoenix; Cat# H-003-57; dilution 1:4,000), Goat anti-TdTomato (SICGEN, Cat# AB8181-200; dilution 1:4,000) and Rabbit anti-PSD-95 (ProteinTech; Cat# 20,665-1-AP; dilution 1:100) mixed in SignalStain® Antibody Diluent (CellsSignal; Cat# 8112) for 48 h at 4 °C plus 2 h at room temperature. ..

    Control:

    Article Title: Molecular profiling of the hippocampus of children with autism spectrum disorder.
    Article Snippet: Blocking was carried out using Biorad EveryBlot blocking buffer with 0.01% Tween-20 (Biorad, Hercules, CA). .. The membranes were probed with the following primary antibodies: rabbit anti-MMP9 (1:1000 μl, cat#AB13458, MilliporeSigma, Burlington, MA), rabbit anti-PSD-95 (1:500 μl, cat#20665-1-AP, Protein Tech, Rosemont, IL), rabbit anti-SYN1 (1:500 μl, cat#20258-1-AP, Protein Tech, Rosemont, IL), rabbit anti-MEF2C (1:500 μl, cat#10056-1-AP, Abnova, Taipei City, Taiwan), DGCR6 (1:500 μl, cat#H00008214-B01P, Protein Tech, Rosemont, IL) using VCP for the loading control (mouse anti-VCP, Santa Cruz Biotechnology cat# sc-57492 and rabbit anti-VCP, Abcam Inc. cat# ab111740) according to prior studies [50]. .. All proteins were visualized with Biorad antimouse Starbright blue 520 and anti-rabbit Starbright blue 700 fluorescence secondary antibodies (Biorad, Hercules, CA).

    Article Title: Molecular profiling of the hippocampus of children with autism spectrum disorder
    Article Snippet: Blocking was carried out using Biorad EveryBlot blocking buffer with 0.01% Tween-20 (Biorad, Hercules, CA). .. The membranes were probed with the following primary antibodies: rabbit anti-MMP9 (1:1000 μl, cat#AB13458, MilliporeSigma, Burlington, MA), rabbit anti-PSD-95 (1:500 μl, cat#20665-1-AP, Protein Tech, Rosemont, IL), rabbit anti-SYN1 (1:500 μl, cat#20258-1-AP, Protein Tech, Rosemont, IL), rabbit anti-MEF2C (1:500 μl, cat#10056-1-AP, Abnova, Taipei City, Taiwan), DGCR6 (1:500 μl, cat#H00008214-B01P, Protein Tech, Rosemont, IL) using VCP for the loading control (mouse anti-VCP, Santa Cruz Biotechnology cat# sc-57492 and rabbit anti-VCP, Abcam Inc. cat# ab111740) according to prior studies [ ]. .. All proteins were visualized with Biorad anti-mouse Starbright blue 520 and anti-rabbit Starbright blue 700 fluorescence secondary antibodies (Biorad, Hercules, CA).

    Western Blot:

    Article Title: The effect of paeoniflorin on the rewarding effect of methamphetamine and the associated cognitive impairment in mice.
    Article Snippet: 1 School of Public Health, Health Science Center, Ningbo University, Ningbo 315211, China 2 National Narcotics Laboratory Zhejiang Regional Center, Hangzhou, China 3 Department of Psychology, Collage of Teacher Education, Ningbo University, Ningbo, China 4 School of Pharmacy, Health Science Center, Ningbo University, Ningbo 315211, China Abstract Chronic exposure to methamphetamine (METH) has been suggested to cause METH use disorder and severe cognitive impairment.. Paeoniflorin (PF) is a monoterpenoid glycoside with various beneficial effects, including anti-inflammatory, antioxidant and antidepressant.. The current study was designed to investigate the effect of PF (30 mg/kg, i.p.) on the rewarding effect of METH (2.5 mg/kg, i.p.) and the associated cognitive impairment, using the animal model of conditioned place preference, new location reorganization test, new object reorganization test and Y-maze test.

    Article Title: The effect of paeoniflorin on the rewarding effect of METH and the associated cognitive impairment in mice
    Article Snippet: .. The following antibodies were used for western blotting: Rabbit anti-PSD-95 (1:1000; 20665-1-AP, Proteintech), mice anti-beta Actin (1:8000; 20536-1-AP, Proteintech), mice anti-SYP (1: 2000; sc-17750, Santa Cruz), Goat anti-Mouse (horseradish peroxidase, HRP), Goat anti-Rabbit (HRP). ..



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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Image Search Results


    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.

    Journal: Frontiers in Endocrinology

    Article Title: Dorzagliatin shows potential in preventing cognitive impairment in diabetes: evidence from Mendelian randomization analysis and animal study

    doi: 10.3389/fendo.2025.1755359

    Figure Lengend Snippet: Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.

    Article Snippet: The membranes were blocked with 1×TBST and 5% BSA (tank blotting) or 5% skim milk (Semi-dry blotting) for 1 hour at room temperature and then incubated overnight at 4°C with following primary antibodies, respectively: mouse monoclonal anti-GluN1 antibody (Millipore, Cat. No: 05-432); rabbit polyclonal anti-GluN2A antibody (NOVUS, Cat. No: NB300-105); rabbit monoclonal anti-GLUT1 antibody (Abcam, Cat. No: ab115730); rabbit polyclonal anti-GLUT3 antibody (Bioss, Cat. No: bs-1207R); rabbit polyclonal anti-IR antibody (Abcam, Cat. No: ab137747); rabbit monoclonal anti-PSD95 antibody (Abcam, Cat. No: ab238135).

    Techniques: Western Blot, Control, Two Tailed Test